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KMID : 0357119950170010065
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Korean Journal of Immunology
1995 Volume.17 No. 1 p.65 ~ p.72
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An Immunoglobulin Light Chain Variable Region Gene of a B-Cell Line Derived form the Rheumatoid Synovium
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손정원
김병호/정동준/라성민/류총근
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Abstract
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The variable of an immunoglobulin (Ig) k light chain gene of a rheumatoid synbovial B-cell line was investigated. The synovial B-cell line. R12J, was generated by EBV transformation and a subsequent fusion with Sp2/0-Ag8 mouse myeloma cells. The
antibody produced by R12J reacted to several antigens such as PPD (purified protein derivative), collagen, DNA and the Fc fragment of human IgG. The Vκsequence shows 99% homology to the germline Vdg gene of a VκIIIa subgroup and the sequence of
a
joining segment is identical to Jκ2. Thus it is most likely that the three nucleotide differences between the germline Vg gene and the variable region of the R12Jκlight chain are generated as a result of somatic hypermutation. All of the
mutations are
located in the CDR1, two of them are in the codon 30 and one in the codon 31, resulting in amino acid changes in both of the codons. An interesting finding is that the CDR3 is comprised of 10 amino acids due to an extra proline residue at the
junction
of the Vκgene and the joining segment. The nature and distribution of the distribution of the mutations and the presence of an extra proline residue at the V-J junction indicate that these antibodies have been subjected to the pressure of
antigenic
selection.
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